Lab-grown kidneys work in animals

BBC, LONDON: Scientists say they are a step closer to growing fully functioning replacement kidneys, after promising results in animals.
When transplanted into pigs and rats, the kidneys worked, passing urine just like natural ones.
Getting the urine out has been a problem for earlier prototypes, causing them to balloon under the pressure.
The Japanese team got round this by growing extra plumbing for the kidney to stop the backlog, PNAS reports.
Although still years off human trials, the research helps guide the way towards the end goal of making organs for people, say experts.
In the UK, more than 6,000 people are waiting for a kidney - but because of a shortage of donors, fewer than 3,000 transplants are carried out each year.
More than 350 people die a year, almost one a day, waiting for a transplant.
Lab-grown kidneys using human stem cells could solve this problem.
Dr Takashi Yokoo and colleagues at the Jikei University School of Medicine in Tokyo used a stem cell method, but instead of just growing a kidney for the host animal, they set about growing a drainage tube too, along with a bladder to collect and store the urine.
When they connected this to the animal's existing bladder, the system worked.
Urine passed from the transplanted kidney into the transplanted bladder and then into the rat bladder.
And the transplant was still working well when they checked again eight weeks later.
They then repeated the procedure on a much larger mammal - a pig - and achieved the same results.
Prof Chris Mason, an expert in stem cells and regenerative medicine at University College London, said: "This is an interesting step forward. The science looks strong and they have good data in animals.
"But that's not to say this will work in humans. We are still years off that. It's very much mechanistic. It moves us closer to understanding how the plumbing might work.
"At least with kidneys, we can dialyse patients for a while so there would be time to grow kidneys if that becomes possible."
Other scientists have looked at rejuvenating old organs that would not normally be suitable for transplanting. Prof Harald Ott and colleagues have been testing out a method that washes away the tissue from dead organs to leave a scaffold that can be repopulated with healthy new cells.
They have built kidneys, hearts and lungs in this way.
Prof Ott says using a scaffold is a good short cut, rather than having to grow whole structures from scratch.
Researchers at Massachusetts General Hospital have taken the first steps towards creating usable engineered kidneys. They took a rat kidney and used a detergent to wash away the old cells.
The remaining web of proteins, or scaffold, looks just like a kidney, including an intricate network of blood vessels and drainage pipes.
This protein plumbing was used to pump the right cells to the right part of the kidney, where they joined with the scaffold to rebuild the organ.
It was kept in a special oven to mimic the conditions in a rat's body for the next 12 days.
When the kidneys were tested in the laboratory, urine production reached 23% of natural ones. The team then tried transplanting an organ into a rat. Once inside the body, the kidney's effectiveness fell to 5%. Yet the lead researcher, Dr Harald Ott, told the BBC that restoring a small fraction of normal function could be enough: "If you're on haemodialysis then kidney function of 10% to 15% would already make you independent of haemodialysis. It's not that we have to go all the way."

 

Lab-grown kidneys work in animals

BBC, LONDON: Scientists say they are a step closer to growing fully functioning replacement kidneys, after promising results in animals. When transplanted into pigs and rats, the kidneys worked, passing urine just like natural ones. Getting the urine out has been a problem for earlier prototypes, causing them to balloon under the pressure. The Japanese team got round this by growing extra plumbing for the kidney to stop the backlog, PNAS reports. Although still years off human trials, the research helps guide the way towards the end goal of making organs for people, say experts. In the UK, more than 6,000 people are waiting for a kidney - but because of a shortage of donors, fewer than 3,000 transplants are carried out each year. More than 350 people die a year, almost one a day, waiting for a transplant. Lab-grown kidneys using human stem cells could solve this problem. Dr Takashi Yokoo and colleagues at the Jikei University School of Medicine in Tokyo used a stem cell method, but instead of just growing a kidney for the host animal, they set about growing a drainage tube too, along with a bladder to collect and store the urine. When they connected this to the animals existing bladder, the system worked. Urine passed from the transplanted kidney into the transplanted bladder and then into the rat bladder. And the transplant was still working well when they checked again eight weeks later. They then repeated the procedure on a much larger mammal - a pig - and achieved the same results. Prof Chris Mason, an expert in stem cells and regenerative medicine at University College London, said: This is an interesting step forward. The science looks strong and they have good data in animals. But thats not to say this will work in humans. We are still years off that. Its very much mechanistic. It moves us closer to understanding how the plumbing might work. At least with kidneys, we can dialyse patients for a while so there would be time to grow kidneys if that becomes possible. Other scientists have looked at rejuvenating old organs that would not normally be suitable for transplanting. Prof Harald Ott and colleagues have been testing out a method that washes away the tissue from dead organs to leave a scaffold that can be repopulated with healthy new cells. They have built kidneys, hearts and lungs in this way. Prof Ott says using a scaffold is a good short cut, rather than having to grow whole structures from scratch. Researchers at Massachusetts General Hospital have taken the first steps towards creating usable engineered kidneys. They took a rat kidney and used a detergent to wash away the old cells. The remaining web of proteins, or scaffold, looks just like a kidney, including an intricate network of blood vessels and drainage pipes. This protein plumbing was used to pump the right cells to the right part of the kidney, where they joined with the scaffold to rebuild the organ. It was kept in a special oven to mimic the conditions in a rats body for the next 12 days. When the kidneys were tested in the laboratory, urine production reached 23% of natural ones. The team then tried transplanting an organ into a rat. Once inside the body, the kidneys effectiveness fell to 5%. Yet the lead researcher, Dr Harald Ott, told the BBC that restoring a small fraction of normal function could be enough: If youre on haemodialysis then kidney function of 10% to 15% would already make you independent of haemodialysis. Its not that we have to go all the way.